dbGaP Job Submission

All dbGaP data is stored on Isilon share mounted automatically when a user requests an interactive dbGaP session.

Step 1: Request an interactive session on Oscar on the dbGaP.

interact -q dbgap -n 20 -m 20g -t 01:00:00

Group membership fordbgap, dbg_PiLastName, dbg_import, dbg_export along with SLURM associations are required for working with dbGaP.

Step 2: Understand the dbGaP Data Hierarchy

The native GPFS file system (Home, Scratch, Data, Runtime) will be read-only access. All dbGaP related work must be done in the/dbGaProot directory. The /dbGaP directory has two sub-directories data & results . The downloaded datasets from the xfer server will be written to/dbgap/import/userand all output files will be written to the/dbgap/results/usernamedirectory. The hierarchy structure is:

psaluja@node1030:/dbGaP$ tree /dbGaP/
/dbGaP/
├── data
│   └── import
│       ├── group_1
│       ├── group_2
│       ├── user_1
|       |   ├── SRR10859003_1.fastq.gz
│       └── user_2
│           ├── SRR10859003_1.fastq.gz
│           ├── SRR10859003_2.fastq.gz
│           └── SRR10859003_3.fastq.gz
└── results
    ├── user_1
    │   ├── job_script.sh
    │   ├── slurm-145960.out
    │   └── slurm-1445969.out
    └── user_2

Step 3: Submitting a dbGaP batch jobs

Home, Scratch & Data will be in read-only mode only users must write their code files, batch scripts in their designated. Example batch script for dbGaP jobs

#!/bin/bash

# Request an hour of runtime:
#SBATCH --time=1:00:00

# Define Partition
#SBATCH -p dbgap

# Use 2 nodes with 8 tasks each, for 16 MPI tasks:
#SBATCH -N 2
#SBATCH --tasks-per-node=8

# Specify a job name:
#SBATCH -J dbGAP_analysis

# Specify an output file
#SBATCH -o /dbGaP/results/psaluja/slurm-%j.out
#SBATCH -e /dbGaP/results/psaluja/slurm-%j.err

# Run a command
module load sratoolkit/2.11.0
srun --mpi=pmix fasterq-dump --ngc your_file.ngc SRR1234567.sra

Any output files including SLURM out and err files must be written to /dbGaP/results directory.